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Literature summary extracted from

  • Le Nours, J.; Ryttersgaard, C.; Lo Leggio, L.; Ostergaard, P.R.; Borchert, T.V.; Christensen, L.L.H.; Larsen, S.
    Structure of two fungal beta-1,4-galactanases: Searching for the basis for temperature and pH optimum (2003), Protein Sci., 12, 1195-1204.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.89
-
 Humicola insolens
3.2.1.89
-
 Thermothelomyces heterothallicus
3.2.1.89
-
 Aspergillus aculeatus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.89 hanging drop vapor diffusion method Humicola insolens
3.2.1.89 hanging drop vapor diffusion method Thermothelomyces heterothallicus
3.2.1.89 hanging drop vapor diffusion method Aspergillus aculeatus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.89 A90S/H91D decreased pH optimum Thermothelomyces heterothallicus
3.2.1.89 D128N increased pH optimum Aspergillus aculeatus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.89 Aspergillus aculeatus
-
-
-
3.2.1.89 Humicola insolens
-
-
-
3.2.1.89 Thermothelomyces heterothallicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.89
-
 Humicola insolens
3.2.1.89
-
 Thermothelomyces heterothallicus
3.2.1.89
-
 Aspergillus aculeatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.89 azurine cross-linked galactan + H2O enzyme releases blue color Aspergillus aculeatus ?
-
 ?
3.2.1.89 azurine cross-linked lupin-galactan + H2O enzyme releases blue color Humicola insolens ?
-
 ?
3.2.1.89 azurine cross-linked lupin-galactan + H2O enzyme releases blue color Thermothelomyces heterothallicus ?
-
 ?
3.2.1.89 beta-1,4-galactan + H2O enzyme releases sugars from galactan Humicola insolens ?
-
 ?
3.2.1.89 beta-1,4-galactan + H2O enzyme releases sugars from galactan Thermothelomyces heterothallicus ?
-
 ?
3.2.1.89 beta-1,4-galactan + H2O enzyme releases sugars from lupin-galactan Aspergillus aculeatus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.89 37
-
 at pH 6.5 Aspergillus aculeatus
3.2.1.89 50
-
 at pH 3.5 Aspergillus aculeatus
3.2.1.89 65
-
-
 Humicola insolens
3.2.1.89 65
-
-
 Thermothelomyces heterothallicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.89 3.5
-
 wild-type enzyme Aspergillus aculeatus
3.2.1.89 4.5
-
 D128N mutant Aspergillus aculeatus
3.2.1.89 6.5
-
 A90S/H91D mutant Thermothelomyces heterothallicus
3.2.1.89 7.5
-
 wild-type enzyme Thermothelomyces heterothallicus
3.2.1.89 8.5
-
-
 Humicola insolens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.89 3 5.5 at least 50% activity Aspergillus aculeatus
3.2.1.89 5.5 8.5 at least 50% activity Thermothelomyces heterothallicus
3.2.1.89 6.5 9.5 at least 50% activity Humicola insolens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.89 2.5 5.5 at least 50% activity after 2 h at 37°C Aspergillus aculeatus
3.2.1.89 3.5 8.5 at least 50% activity after 2 h at 37°C Humicola insolens